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KMID : 0624620090420070450
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BMB Reports
2009 Volume.42 No. 7 p.450 ~ p.455
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Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (triticum aestivum) FA85
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Hou Dian-yun
Xu Hong
Du Guang-yuan
Duan Min
Guo Ai-guang
Lin Jun-tang
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Abstract
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The “stage albinism line of winter wheat” FA85 was a specific natural mutant strain on leaf color. This physiological mutation was controlled by cytogene. In order to reveal the genetic and biochemical mechanism of albinism, 2-DE was used to investigate the difference of chloroplast protein expression pattern between FA85 and its parent wheat Aibian 1. From the results of 2-DE gels analysis, approximately 683 spots were detected on each gel, and 57 spots were expressed differently at least two-fold. Using MALDI-TOF/TOF MS, 14 of 57 spots were identified, which could be categorized into four classes: carbon metabolism, energy metabolism, defense/stress response and signal transduction. Compared with the parent wheat, the expression of ATPase-γ and GP1-α was up-regulated in FA85, and of other proteins was down-regulated. Together, we concluded that the expression of chloroplast proteins had changed obviously in FA85, which might be related to the leaf color mutant.
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KEYWORD
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Chloroplast proteins, FA85 mutant, MALDI-TOF/TOF MS, Proteome, 2-DE gel
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